Please use this to share protocols of interest to you. Provide a purpose to the article and, if possible, comment on the role of the major ingredient(s) in your protocol. Thank you!
I would like to share a protocol from Sawada et al. Current Neurobiology 2010 1(1): 21-24 for the heat induced antigen retrieval on whole mount immunostaining of the adult mouse cerebellum.
The beauty of this protocol is that it shows positive staining of two low-signal antibodies in dendrites of specific subsets of Purkinje cells. The overall achievement of this protocol is that it permits the investigator to skip the chilling and thawing processes for antigen retrieval.
Also, antibody penetration is achieved at a higher magnitude, more than 200 μm from the pial surface of the cerebellar cortex.
Thus, heat induced antigen retrieval is a novel technique that permits the visualization of low signal antigens in whole mount tissue.
Sawada K. and Sun Xue-Zhi. Current Neurobiology 2010; 1 (1): 21-24
I would like to share a protocol from Sawada et al. Current Neurobiology 2010 1(1): 21-24 for the heat induced antigen retrieval on whole mount immunostaining of the adult mouse cerebellum.
ReplyDeleteThe beauty of this protocol is that it shows positive staining of two low-signal antibodies in dendrites of specific subsets of Purkinje cells. The overall achievement of this protocol is that it permits the investigator to skip the chilling and thawing processes for antigen retrieval.
Also, antibody penetration is achieved at a higher magnitude, more than 200 μm from the pial surface of the cerebellar cortex.
Thus, heat induced antigen retrieval is a novel technique that permits the visualization of low signal antigens in whole mount tissue.
Sawada K. and Sun Xue-Zhi. Current Neurobiology 2010; 1 (1): 21-24